Basic and Clinical Science Course, 2008-2009, Section 4: by Debra J. Shetlar, Patricia Chevez-Barrios, Sander Dubovy,

By Debra J. Shetlar, Patricia Chevez-Barrios, Sander Dubovy, Robert H. Rosa, Jr, Nasreen Syed, Matthew W. Wilson, Ron W. Pelton, Jacob Pe'er

Discusses advances within the analysis and type of tumors because it publications the reader via a logical, tissue-specific series that levels from topography via disorder strategy to common and differential analysis. Many new colour pathologic and scientific photos and diagrams spotlight this most modern revision. Covers wound fix; specimen dealing with, together with processing and marking; and diagnostic suggestions. additionally features a list for soliciting for ophthalmic pathologic session.

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Additional info for Basic and Clinical Science Course, 2008-2009, Section 4: Ophthalmic Pathology and Intraocular Tumors

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Formation of proliferative intraocular membranes may 30 . Ophthalmic Pathology and Intraocular Tumors A B Figure 2-" Focal posttraumatic choroidal granulomatous inflammation. A, Enucleated eye with a projectile causing a perforating limbal injury that extends to the posterior choroid. B, Microscopic examination shows a focus of choroidal granulomatous inflammation. (Courtesy of Hans E. Grossniklaus. ) affect the timing of vitreoretinal surgery. The timing of the drainage of a ciliochoroidal hemorrhage is based on lysis of the blood clot (10-14 days).

Ample amount of 10% formalin (at least 5 times the size of the biopsy) b. label with patient's name and location of biopsy 3. Drawing/map of site of biopsy for orientation of margins (eyelid lesions for margins, en bloc resections of conjunctiva, scleral, and ciliary body/iris tumors) Frozen Sections 1. If possible, previous communication with ophthalmic pathologist 2. Fill out specific frozen section requisition form, specifying the reason for submitting tissue, such as a. Margins b. Diagnosis c.

Deleted regions = red with ratio <1. Amplified regions = green with ratio> 1. Differentially labeled test and reference DNAs, hybridized to cloned fragments, genomic DNA or cDNA, which are spotted on a glass slide (the array). The DNA copy number aberrations measured by detecting intensity differences in the hybridization patterns 2. Analyzes all chromosomes in a single experiment and no dividing cells required High resolution Disadvantages 1. Inability to detect aberrations not resulting in copy number changes 2.

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Basic and Clinical Science Course, 2008-2009, Section 4: by Debra J. Shetlar, Patricia Chevez-Barrios, Sander Dubovy,
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