Animal cell technology: from biopharmaceuticals to gene by Leda R. Castilho

By Leda R. Castilho

Advent to animal mobilephone know-how /, Animal cells: easy ideas /, Cloning and expression of heterologous proteins in animal cells /, cellphone metabolism and its keep an eye on in tradition /, tradition media for animal cells /, Post-translational amendment of recombinant proteins /, Mechanisms of mobilephone proliferation and telephone demise in animal mobile tradition in vitro /, Mathematical types for progress and product synthesis in animal cellphone tradition /, Bioreactors for animal cells /, tracking and keep an eye on of mobile cultures /, Animal phone separation /, Product purification approaches /, quality controls of biotechnological items /, Regulatory features /, highbrow estate /, Recombinant healing proteins /, Monoclonal antibodies /, Viral vaccines: options, ideas, and bioprocesses /, Bioinsecticides /, telephone remedies and stem cells /, Gene remedy /; Paula Marques Alves, Manuel Jose Teixeira Carrondo, & Pedro Estilita Cruz --, Patricia Leo ... [et al.] --, Mariela Bollati-Fogolin & Marcelo A. Comini --, Paola Amable & Michael Butler --, Angela Maria Moraes, Ronaldo Zucatelli Mendonca, & Claudio Alberto Torres Suazo --, Michael Butler --, Maira Peixoto Pellegrini, Rodrigo Coelho Ventura Pinto, & Leda dos Reis Castilho --, Elisabeth F.P. Augusto, Manuel F. Barral, & Rosane A.M. Piccoli --, Ernesto Chico Veliz, Gryssell Rodriguez, & Alvio Figueredo Cardero --, Aldo Tonso --, Leda dos Reis Castilho & Ricardo de Andrade Medronho --, Angela Maria Moraes, Leda Castilho, & Sonia Maria Alves Bueno --, Marina Etcheverrigaray & Ricardo Kratje --, Maria Teresa Alves Rodrigues & Ana Maria Moro --, Ana Cristina Almeida Muller & Leila Costa Duarte Longa --, Maria Candida Maia Mellado & Leda dos Reis Castilho --, Wirla M.S.C. Tamashiro & Elisabeth F.P. Augusto --, Isabel Maria Vicente Guedes de Carvalho Mello ... [et al.] --, Marcia Regina da Silva Pedrini & Ronaldo Zucatelli Mendonca --, Hamilton da Silva Jr. & Radovan Borojevic --, Celio Lopes Silva ... [et al.]

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This type of vaccine is discussed more extensively in Chapter 18. For gene therapy, hematopoietic stem cells are the most common targets due to their capacity for expansion and differentiation. The current applications focus essentially on diseases caused by a localized genetic deficiency. , 1993). In these cases, the progenitor cells transformed with the respective functional genes complement the immune system of patients. See Chapter 21 for a more extensive discussion on gene therapy. 5 Applications of animal cells in the development of new products The majority of the products currently in use are formed by chemical agents that can be potentially dangerous for human health.

Biotechnol. 23:857–861. Cavazzana-Calvo M, Hacein-Bey S, Basile GS, Gross F, Yvon E, Nusbaum P, Selz F, Hue C, Certain S, Casanova J-L, Bousso P, Le Deist F, Fisher A (2000), Gene therapy of human severe combined immunodeficiency (SCID)-X1 disease, Science 288:669–672. Cruz PE, Maranga L, Carrondo MJT (2002), Integrated process optimisation: lessons from retrovirus and virus like production, J. Biotechnol. 99:199–214. Daley GQ (2003), From embryos to embryoid bodies: generating blood from embryonic stem cells, Ann.

All these developments permitted the practical, efficient, and robust culture of animal cells in bioreactors at scales larger than 10 m3 for consistently producing safe and effective biopharmaceuticals. In the present book, Animal Cell Technology: From Biopharmaceuticals to Gene ˆ ngela M. Moraes, Elisabeth F. Pires Augusto and Therapy, editors Drs Leda R. Castilho, A Michael Butler have integrated the background knowledge and developments of the last 20 years that have converted the culture of animal cells into a vigorous and important scientific field.

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Animal cell technology: from biopharmaceuticals to gene by Leda R. Castilho
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